بررسی اثر عصاره برگ رامنوس فرانگولا میلر بر توانایی بقای سلول های سرطانی سینه (MCF-7) در محیط کشت

Background: Various studies have shown that plant extracts may have cytotoxic effects on cancer cells. The aim of this study was to determine the effects of Rhamnus frangula miller leaf on viability of breast cancer cells in cell culture. Methods: In this laboratory experimental study breast cancer cell line (MCF-7) was used. MCF cells were cultured in RPMI 1640 culture medium containing 10% fetal bovine serum and 100 unit/ml penicillin and 100 &micro;g/ml streptomycin and were kept in incubator at 37 C and at pressure of 5% CO2. The cells were randomly divided into control group and groups exposed to 10, 1, 0.1, 0.01 and 0.001 mg/ml of Rhamnus frangula leaf extract. Viability of cells was assayed using MTT test. The data were analyzed using ANOVA. Results: There was no significant difference between viability of MCF7 cells in group exposed to 0.001 mg/ml of extract and control group. However, viability of MCF7 cells significantly decreased in groups exposed to 0.01, 0.1, 1 and 10 mg/ml of extract compared with control group (p<0.05). Conclusion: Our findings indicated that low dose of the Rhamnus frangula leaf extract has no significant cytotoxic effect on MCF-7 cells. However, high doses of extract of have cytotoxic effects on MCF-7 cells. &nbsp;